pcdna3 ccne2 vector  (Addgene inc)

Journal: Translational Oncology

Article Title: Specific Pomegranate Juice Components as Potential Inhibitors of Prostate Cancer Metastasis 1 2

doi:

Figure Lengend Snippet: Effect of L + E + P on genes involved in cell growth, cell adhesion, and migration of hormone-independent prostate cancer cells. (A) Schematic summary of the effects of L + E + P on gene and miRNA expression in hormone-independent prostate cancer cells. (B) The mRNA levels of CCNE2, ANLN, CCNB1, DTL, HMMR, TWIST, ROCK2, CDC25B, BCL2, NEXN, CDK6, COL1A1, FSCN1, EZH2, PTEN, CDKN1A, and CLDN1 were determined by using qPCR with RNA extracted from PC3 cells treated with L + E + P at 8 µg/ml for 12 hours.

Article Snippet: PC3 cells (80–90% confluent) were transfected with Lipofectamine 2000 (Invitrogen, Carlsbad, CA) following the manufacturer's protocols; 40 nM E-cadherin chimera small-interfering RNA (siRNA) (Abnova, Taiwan, China) was transfected, and 2 µg/ml pcDNA3.1 HMMR vector, pcDNA4.1 TWIST vector, or pcDNA3 CCNE2 vector (Addgene plasmid 19935; Addgene, Cambridge, MA) was transfected.

Techniques: Migration, Expressing

Journal: Translational Oncology

Article Title: Specific Pomegranate Juice Components as Potential Inhibitors of Prostate Cancer Metastasis 1 2

doi:

Figure Lengend Snippet: Gene Analysis of the Effects of L + E + P on PC3 Cells.

Article Snippet: PC3 cells (80–90% confluent) were transfected with Lipofectamine 2000 (Invitrogen, Carlsbad, CA) following the manufacturer's protocols; 40 nM E-cadherin chimera small-interfering RNA (siRNA) (Abnova, Taiwan, China) was transfected, and 2 µg/ml pcDNA3.1 HMMR vector, pcDNA4.1 TWIST vector, or pcDNA3 CCNE2 vector (Addgene plasmid 19935; Addgene, Cambridge, MA) was transfected.

Techniques: Migration, Binding Assay

Journal: Translational Oncology

Article Title: Specific Pomegranate Juice Components as Potential Inhibitors of Prostate Cancer Metastasis 1 2

doi:

Figure Lengend Snippet: Effect of L + E + P on Cancer-Related miRNAs.

Article Snippet: PC3 cells (80–90% confluent) were transfected with Lipofectamine 2000 (Invitrogen, Carlsbad, CA) following the manufacturer's protocols; 40 nM E-cadherin chimera small-interfering RNA (siRNA) (Abnova, Taiwan, China) was transfected, and 2 µg/ml pcDNA3.1 HMMR vector, pcDNA4.1 TWIST vector, or pcDNA3 CCNE2 vector (Addgene plasmid 19935; Addgene, Cambridge, MA) was transfected.

Techniques:

Journal: Translational Oncology

Article Title: Specific Pomegranate Juice Components as Potential Inhibitors of Prostate Cancer Metastasis 1 2

doi:

Figure Lengend Snippet: Mechanistic study of the effect of L + E + P on cell growth, cell adhesion, and migration of hormone-independent prostate cancer cells. (A) PC3 cells were transfected with pcDNA3 CCNE2 vector (2 µg/ml) and treated with L + E + P at 8 µg/ml 24 hours after transfection. Cell number was counted at a 48-hour time point. (B) Immunoblot analysis for E-cadherin with protein extracts from PC3 cells treated with L + E + P at 8 µg/ml for 24 hours. (C) PC3 cells were transfected with 40 nM E-cadherin siRNA. Twenty-four hours after transfection, cells were treated with L + E + P at 8 µg/ml for 12 hours and adhesion assay was performed. PC3 cells were transfected with 2 µg/ml (D) pcDNA3.1 HMMR vector or (E) pcDNA4.1 TWIST vector and treated with L + E + P at 8 µg/ml 24 hours after transfection. Migrated distance was determined at a 48-hour time point. Bars represent SEM. **P < .01; *P < .05.

Article Snippet: PC3 cells (80–90% confluent) were transfected with Lipofectamine 2000 (Invitrogen, Carlsbad, CA) following the manufacturer's protocols; 40 nM E-cadherin chimera small-interfering RNA (siRNA) (Abnova, Taiwan, China) was transfected, and 2 µg/ml pcDNA3.1 HMMR vector, pcDNA4.1 TWIST vector, or pcDNA3 CCNE2 vector (Addgene plasmid 19935; Addgene, Cambridge, MA) was transfected.

Techniques: Migration, Transfection, Plasmid Preparation, Western Blot, Cell Adhesion Assay